Maestría en Bioquímica
Permanent URI for this collection
Browse
Browsing Maestría en Bioquímica by Subject "540"
Now showing 1 - 3 of 3
Results Per Page
Sort Options
Item Genes asociados a la síntesis y resistencia de compuestos antimicrobianos en islas de recursos de un ambiente semiárido del Caribe Colombiano.(Universidad Antonio Nariño) Camargo Montoya, Martha Liliana; Vanegas Guerrero, Javier; Arenas Suárez, Nelson EnriqueThe phylum Actinobacteria is of great importance for its capacity for biosynthesis and resistance of antimicrobial compounds and dominance in soil microbiomes. It is considered that unexplored and extreme environments such as resource islands in arid and semi-arid zones of the tropics can be a source of a great diversity of Actinobacteria and therefore of potential compounds of biotechnological interest. The objective of this study was to determine the effect of seasonality and the presence of vegetation on the abundance of actinobacteria and genes associated with the synthesis and resistance of antimicrobial compounds associated with three resource islands in a semiarid region of the Colombian Caribbean. For this purpose, three nurse trees Prosopis juliflora, Haematoxylum brasiletto and Pithecellobium dulce were sampled under canopy (V), bare soil (C) both in wet (W) and dry season (D). Total DNA was extracted and sequenced. From the sequences, 16 orders, 28 families and 52 genera of actinobacteria with higher abundances in CD were detected. In addition, 16 antimicrobial compound biosynthesis pathways were detected, of which the synthesis pathways of monobactam antibiotics, prodigiosin and streptomycin were the most abundant. These pathways showed higher abundances in DV. Ten antibiotic resistance pathways were detected including the highest abundances for rifamycin in CD and streptogramins (MLS) in VW. It was observed that the diversity and abundance of actinobacterial biosynthesis and resistance genes were influenced by seasonality and the presence of vegetation in the resource islands.Item Genómica comparativa de aislamientos clínicos Colombianos de Salmonella Typhimurium Variante Monofásica(Universidad Antonio Nariño) Cuenca Arias, Paloma; Wiesner Reyes, Magdalena; Arenas Suárez, Nelson EnriqueSalmonella Typhimurium Monophasic variant (STVM), has become a problem in public health globally due to its sudden rise of dominant clones and fast spread. In Colombia, the National Institute of Health reported the circulation of this variant since 2015, ranked sixth among the 20 Salmonella serovars isolated with the highest frequency. Our aim was to characterize genotypically the STVM Colombian isolates confirming the clone-type spreading nationwide. In this study, we included a whole-genome sequencing dataset of 21 Colombian clinical isolates of STVM that were analyzed by using bioinformatics and comparative genomics tools. Our results suggest high genomic plasticity in STVM strains mediated by the acquisition of mobile and conjugative genetic elements, conferring heavy metal tolerance and antibiotic resistance. Moreover, the absence of genes involved in adhesion, invasion, and colonization processes suggest an adaptation process of the new serovar, independently of clonal lineage, source, or geographical origin.Item “Interacción entre la proteína de resistencia de yuca RXAM2 y efectores de Xanthomonas phaseoli pv. manihotis”(Universidad Antonio Nariño) Silva Fernández, Lesly Ximena; Díaz Tatis, Paula Alejandra; Reyes Guzmán, Edwin AlfredoThe Cassava bacterial blight is a disease caused by the bacterium Xanthomonas phaseoli pv. manihotis (Xpm) and has been reported in all regions of the world where cassava is cultivated. The most economical and effective strategy for the management of the disease is the use of resistant varieties, but there have been few studies aimed at understanding the molecular basis of the resistance of cassava to this pathogen. Recently it was identified that the NLR protein named RXM2 confers resistance to different strains of Xpm. However, the molecular mechanism used by this protein to recognize Xpm effectors is unknown. This project allowed the generation of the possible three-dimensional structure of RXAM2 and XopE1, XopE4, XopC2, XopAK and XopV effectors of Xpm, by homology modeling. The LRR domain was identified as the possible responsible for the recognition of the effectors, through rigid and flexible molecular docking. On the other hand, it was possible to identify sites under negative selection in 65 Xpm strains, which suggests a high conservation between the effectors present in strains from different biogeographic regions.