Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis

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dc.contributor.advisorDíaz Tatis, Paula Alejandraspa
dc.contributor.advisorSanchez Ferro, Juan Sebastianspa
dc.contributor.authorSantamaría Rodríguez, Brian Davidspa
dc.date.accessioned2021-02-22T14:28:01Z
dc.date.available2021-02-22T14:28:01Z
dc.date.issued2020-06-03spa
dc.description.abstractXanthomonas phaseoli pv. manihotis (Xpm) is the causal agent of cassava bacterial blight, a disease that can generate considerable losses in this crop. This bacterium possesses effector proteins that are injected into the host cell through the type-three secretion system and that function as virulence factors. Some of these effectors, called TALEs (Transcription-Activator Like Effectors), enter the nucleus of host cells, bind specifically to host DNA sequences, called effector-binding elements (EBEs), and act as transcription factors to modulate host gene expression. The knowledge of the mode of action for TALEs in species as rice has allowed the development of biotechnological strategies to generate broad-spectrum resistance to pathogens. In this study, a trap promoter was developed that is activated by three predominant TALEs in Xpm strains that allow the expression of the resistance gene RXam2(MHV). Our results show that when TALEs 14, 20 and 22 are co-infiltrated with the synthetic promoter that contains the EBEs for this TALEs a hypersensitive response is produced in the infiltrated area in leaves of N. tabacum. These results represent an important achivement for the use of this strategy in future cassava breeding programs.eng
dc.description.abstractXanthomonas phaseoli pv. manihotis (Xpm) es el agente causal de la bacteriosis vascular de la yuca, enfermedad que puede generar pérdidas considerables en este cultivo. Esta bacteria posee proteínas efectoras que son inyectadas al interior de la célula hospedera a través del sistema de secreción tipo tres y que funcionan como factores de virulencia. Los efectores llamados TALEs (del inglés, Transcription-Activator Like Effector), ingresan al núcleo de las células hospederas, se unen de manera específica a las secuencias de ADN del hospedero, denominadas elementos de unión al efector (EBE) y actúan como factores de transcripción para modular la expresión de genes del hospedero. El conocimiento del modo de acción de los TALEs ha permitido desarrollar estrategias biotecnológicas para generar resistencia de amplio espectro a patógenos de plantas. En este estudio se elaboró un promotor trampa activado por tres TALEs predominantes en cepas de Xpm para inducir la expresión del gen de resistencia RXam2(MHV). Los resultados de los experimentos de expresión transitoria muestran que la co-infiltración de los TALEs 14, 20 y 22 junto con el promotor trampa que contiene los EBEs para estos tres efectores causan una respuesta hipersensible en el área infiltrada en hojas de N.tabacum.spa
dc.description.degreelevelPregradospa
dc.description.degreenameBioquímico(a)spa
dc.description.notesPresencialspa
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dc.identifier.instnameinstname:Universidad Antonio Nariñospa
dc.identifier.reponamereponame:Repositorio Institucional UANspa
dc.identifier.repourlrepourl:https://repositorio.uan.edu.co/spa
dc.identifier.urihttp://repositorio.uan.edu.co/handle/123456789/1600
dc.language.isospaspa
dc.publisherUniversidad Antonio Nariñospa
dc.publisher.campusBogotá - Circunvalarspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.programBioquímicaspa
dc.rightsAcceso abierto
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subjectbacteriosis vascular de la yuca, promotor trampa, respuesta hipersensible.es_ES
dc.subject.keywordcassava bacterial blight, trap promoter, hypersensitive response.es_ES
dc.titleInducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotises_ES
dc.typeTrabajo de grado (Pregrado y/o Especialización)spa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1fspa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
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