Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma

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dc.contributor.advisorLosada Barragán, Mónicaspa
dc.contributor.advisorUpegui Zapata, Yulieth Alexandraspa
dc.contributor.authorGarcia Daza, Dayana Katerinespa
dc.creator.cedula11821827865spa
dc.date.accessioned2023-01-17T17:23:55Z
dc.date.available2023-01-17T17:23:55Z
dc.date.issued2022-12-13spa
dc.description.abstractGlioblastoma is the most aggressive and malignant tumor of the brain, with a survival rate of no more than 12 months. Currently, resistance to treatments has been evidenced, so there is a need to explore adjuvant therapies to those that already exist. Among them is the ketogenic diet based on medium-chain free fatty acids, which has shown antiangiogenic, anti-inflammatory and proapoptotic properties in various diseases. In this study, cell overload, oxidative stress, and the relative expression of PTEN, CCND3, HIF 1α, and BCL2 tumor genes in T98G cells after octanoic and decanoic acid supplementation for 3 days were evaluated. A reduction in cell turnover, superoxide levels and reactive oxygen species was found in both octanoic acid and decanoic acid treated cells. In addition, a reduction of BCL2 in cells with decanoic acid and an increase in the expression of PTEN with low glucose octanoic acid is evidenced, which suggests a recovery in the control of cell death. The study demonstrates the potential in vitro use of medium chain fatty acids as adjuvant therapyeng
dc.description.abstractEl glioblastoma es el tumor más agresivo y maligno del cerebro, con una tasa de supervivencia no mayor a los 12 meses. En la actualidad se ha evidenciado resistencia a los tratamientos, por lo que hay una necesidad de explorar terapias adyuvantes de los ya existentes. Entre los que se encuentra la dieta cetogénica a base de ácidos grasos libres de cadena media que ha demostrado propiedades antiangiogénicas, antiinflamatorias y proapoptóticas en diversas enfermedades. En este estudio se evaluó la proliferación celular, el estrés oxidativo, y la expresión relativa de los genes tumorales PTEN, CCND3, HIF 1α y BCL2 en las células T98G después de la adición de ácido octanoico y decanoico durante 3 días. Se encontró una reducción en la proliferación celular, en los niveles de superóxido y especies reactivas de oxígeno tanto en células tratadas con ácido octanoico como con ácido decanoico. Además, se evidenció una reducción de BCL2 en células con ácido decanoico y un aumento en la expresión de PTEN con ácido octanoico bajo en glucosa lo que sugiere una recuperación en el control de muerte celular. El estudio demuestra el uso potencial in vitro de los ácidos grasos de cadena media como terapia adyuvantespa
dc.description.degreelevelPregradospa
dc.description.degreenameBioquímico(a)spa
dc.description.degreetypeInvestigaciónspa
dc.description.notesPresencialspa
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dc.identifier.instnameinstname:Universidad Antonio Nariñospa
dc.identifier.reponamereponame:Repositorio Institucional UANspa
dc.identifier.repourlrepourl:https://repositorio.uan.edu.co/spa
dc.identifier.urihttp://repositorio.uan.edu.co/handle/123456789/7386
dc.language.isospaspa
dc.publisherUniversidad Antonio Nariñospa
dc.publisher.campusBogotá - Circunvalarspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.programBioquímicaspa
dc.rightsAcceso abierto
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subjectglioblastomaes_ES
dc.subjectdecanoic acides_ES
dc.subjectoctanoic acides_ES
dc.subjectoxidative stresses_ES
dc.subjectproliferationes_ES
dc.subject.ddc572es_ES
dc.subject.keywordglioblastomaes_ES
dc.subject.keyworddecanoic acides_ES
dc.subject.keywordoctanoic acides_ES
dc.subject.keywordoxidative stresses_ES
dc.subject.keywordproliferationes_ES
dc.titleEvaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastomaes_ES
dc.typeTrabajo de grado (Pregrado y/o Especialización)spa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1fspa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audienceEspecializadaspa
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