Evaluación in silico de toxinas peptídicas de origen animal con efecto antagonista sobre la actividad del Receptor - N-Metil-D-Aspatarto (NMDA)

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dc.contributor.advisorReyes Guzman, Edwin Alfredospa
dc.contributor.advisorForero Vivas, María Elisaspa
dc.contributor.authorGamboa Rodríguez, Katherin Alejandraspa
dc.creator.cedula11931826387spa
dc.date.accessioned2021-10-14T16:03:04Z
dc.date.available2021-10-14T16:03:04Z
dc.date.issued2021-06-23spa
dc.description.abstractThe N-methyl-D-aspartate receptor (NMDAR) constitutes the main subtype of glutamate receptors, involved in physiological processes such as neuronal development, transmission and synaptic plasticity, and in numerous pathological conditions such as ischemic damage, chronic pain, psychosis, and other degenerative disorders. The NMDAR has a structural topology consisting of four subunits mainly of the GluN1 and GluN2(A-B) type. The ionotropic NMDAR corresponds to an ionic-cation channel, permeable mainly to calcium ion. An excessive increase in calcium influx via NMDAR generates excitotoxicity which translates into neuronal damage and death. Based on the above, it is essential to search for molecules that generate interaction with the receptor and specifically with the GluN2B subunit, modulate the activity of the receptor-channel and can be recycled or eliminated by the organism. The present thesis proposal proposes the design and in silico characterization of peptides derived from animal toxins with potential targeting of the NMDAR. The design included the use of computational analysis tools, alignments and docking and molecular dynamics simulations, which allowed predicting the three-dimensional structure of a series of peptide toxins and proposing peptides derived from them as potential NMDAR ligandseng
dc.description.abstractEl receptor N-Metil-D-Aspartato (NMDAR) constituye el principal subtipo de receptores de glutamato, implicado en procesos fisiológicos como desarrollo neuronal, transmisión y plasticidad sináptica, y en numerosas condiciones patológicas como el daño isquémico, dolor crónico, psicosis, y otros trastornos degenerativos. El NMDAR presenta una topología estructural conformada por cuatro subunidades principalmente de tipo GluN1 y GluN2(AB). El NMDAR ionotrópico corresponde a un canal iónico-catiónico, permeable principalmente al ion calcio. Un aumento excesivo del influjo de calcio vía NMDAR genera excitotoxicidad la cual se traduce en daño y muerte neuronal. Con base en lo anterior se hace indispensable la búsqueda de moléculas que generen interacción con el receptor y específicamente con la subunidad GluN2B, modulen la actividad del receptor-canal y puedan ser reciclados o eliminados por el organismo. La presente propuesta de tesis plantea el diseño y caracterización in silico de péptidos derivados de toxinas de origen animal como potencial blanco el NMDAR. El diseño incluyó el uso de herramientas computacionales de análisis, alineamientos y simulaciones de docking y dinámica molecular, lo que permitió predecir la estructura tridimensional de una serie de toxinas peptídicas y proponer péptidos derivados de estas como posibles ligandos del NMDARspa
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Bioquímicaspa
dc.description.notesPresencialspa
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dc.identifier.instnameinstname:Universidad Antonio Nariñospa
dc.identifier.reponamereponame:Repositorio Institucional UANspa
dc.identifier.repourlrepourl:https://repositorio.uan.edu.co/spa
dc.identifier.urihttp://repositorio.uan.edu.co/handle/123456789/5036
dc.language.isospaspa
dc.publisherUniversidad Antonio Nariñospa
dc.publisher.campusBogotá - Circunvalarspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.programMaestría en Bioquímicaspa
dc.rightsAcceso abierto
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subjectNMDAes_ES
dc.subjecttoxinases_ES
dc.subjectpéptidoes_ES
dc.subjectexcitotoxicidades_ES
dc.subjectbioinformáticaes_ES
dc.subjectcalcio intracelulares_ES
dc.subject.keywordNMDAes_ES
dc.subject.keywordtoxinses_ES
dc.subject.keywordpeptidees_ES
dc.subject.keywordexcitotoxicityes_ES
dc.subject.keywordbioinformatics intracellular calciumes_ES
dc.titleEvaluación in silico de toxinas peptídicas de origen animal con efecto antagonista sobre la actividad del Receptor - N-Metil-D-Aspatarto (NMDA)es_ES
dc.typeTesis y disertaciones (Maestría y/o Doctorado)spa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
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