Criopreservación de espermatozoides: citoesqueleto, balsas lipídicas e integridad nucleoproteíca
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Date
2021-04-26
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Universidad Antonio Nariño
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http://purl.org/coar/resource_type/c_7a1f
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Abstract
This work aimed to carry out an approach through literature review of the main damages in the
cytoskeleton, plasma membrane lipid rafts and the sperm nucleus caused by cryopreservation
procedures. For this purpose, a search was carried out in different databases: Pub Med, Elsevier,
CAB abstract and Google Scholar, with previously defined keywords. forty-three articles were
obtained, which were analyzed and discussed in the literature review. The alteration in the
cytoskeletal structure of spermatozoa caused by cryopreservation procedures is associated with the
cryocapacitation phenomenon or “premature capacitation” of the same, expressed in increased Factin polymerization. Lipid rafts are microdomains present in the plasma membrane and play an
important role in the regulation of signal transmission in sperm. It has been found that
cryopreservation produces irreversible aggregation of proteins, some of these associated with lipid
rafts and it is hypothesized that the lipid rafts are affected preventing their movement towards the
sperm apical zone, which results in a reduction in the fertilizing ability. Cryopreservation affects
the degree of chromatin condensation, produces DNA fragmentation and changes in important
genes. In conclusion, this review provides important information that allows an alternative
emphasis to be made to the damage of the plasma membrane reviewed by other authors, providing
evidence of the damage to the cytoskeleton, lipid rafts and the sperm nucleus caused by
cryopreservation.