Caracterización de bacteriocinas de Bacillus subtilis ATCC 6633 y Pseudomonas extremaustralis CMPUJ U515 en la perspectiva de control hacía patógenos humanos

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dc.contributor.advisorLópez Pazos, Silvio Alejandro
dc.contributor.advisorRojas Arias, Adriana Carolina
dc.creatorLandinez Velandia, Sandra Milena
dc.date.accessioned2021-02-22T15:28:23Z
dc.date.available2021-02-22T15:28:23Z
dc.date.created2020-05-29
dc.description.abstractBacteriocins comprise a large number of peptides synthesized ribosomically by countless numbers of bacteria. Biosynthesis is carried out by structural genes that encode these peptides. The increasing bacterial resistance threatens human and animal health. Bacillus subtilis has been shown to produce a wide range of bacteriocins. The genus Pseudomonas sp. synthesizes different antimicrobial peptides to dominate over other competing organisms. P. extremeustralis species is a non-pathogenic Gram-negative bacterium, isolated from a pond in the Antarctic Peninsula. Twenty-one bacteriocin sequences were identified in silico in the genome of B. Subtilis ATCC 6633 and two associated sequences in the genome of P. extremeustralis CMPUJ U515. The physicochemical properties allowed establishing the molecular weight, the isoelectric point, the positively and negatively charged residues, the extinction coefficient, the stability index, the aliphatic index and the overall average of hydropaticity (GRAVY). The products obtained from PCR, resulted in three products that correspond to the bacteriocins Subtilin, Sublancin 168 and Subtilocin A. Finally, it was desired to establish the possible growth inhibitory effect of the protein extracts of B. subtilis ATCC 6633 and P. extremaustralis CMPUJ U515, for which a qualitative sensidisk test was performed on the strains of Escherichia coli ATCC 25922 and Staphylococcus aureus CMPUJ 080. Unfortunately no activity was found. In this investigation we concluded that Bacillus subtilis ATCC 6633 has biocontrol potential due to its bacteriocins which can be cloned by homologous recombination into yeast, to analyze its biological functionality.es_ES
dc.description.degreelevelMaestríaes_ES
dc.description.degreenameMagister en Bioquímicaes_ES
dc.description.notesPresenciales_ES
dc.description.tableofcontentsLas bacteriocinas abarcan un gran número de péptidos sintetizados ribosómicamente por innumerable cantidad de bacterias. La biosíntesis es llevada a cabo por genes estructurales que codifican estos péptidos. La creciente resistencia bacteriana amenaza la salud humana y animal. Se ha demostrado que Bacillus subtilis produce amplia gama de bacteriocinas. El género Pseudomonas sp. sintetiza diferentes péptidos antimicrobianas para dominar sobre los otros organismos en competencia. La especie P. extremaustralis es una bacteria Gram negativa no patógena, aislada de un estanque de la Península Antártica. Se identificaron in silico 21 secuencias de bacteriocinas en el genoma de B. Subtilis ATCC 6633 y dos secuencias asociadas en el genoma de P. extremaustralis CMPUJ U515. Las propiedades fisicoquímicas permitieron establecer el peso molecular, el punto isoeléctrico, los residuos cargados positiva y negativamente, el coeficiente de extinción, el índice de estabilidad, el índice alifático y el promedio general de hidropaticidad (GRAVY). Los productos obtenidos de PCR, dio como resultado tres productos que corresponden a las bacteriocinas Subtilina, Sublancina 168 y Subtilocina A. Finalmente se deseaba establecer el posible efecto inhibidor de crecimiento de los extractos proteicos de B. subtilis ATCC 6633 y P. extremaustralis CMPUJ U515, para lo cual se realizó una prueba cualitativa con sensidiscos sobre las cepas de Escherichia coli ATCC 25922 y Staphylococcus aureus CMPUJ 080. Desafortunadamente no se encontró actividad. En esta investigación concluimos que Bacillus subtilis ATCC 6633 tiene potencial biocontrolador debido a sus bacteriocinas las cuales pueden clonarse mediante recombinación homologa en levadura, para analizar su funcionalidad biológica.es_ES
dc.identifier.urihttp://repositorio.uan.edu.co/handle/123456789/1605
dc.language.isospaes_ES
dc.publisherUniversidad Antonio Nariñoes_ES
dc.publisher.campusBogotá - Circunvalar
dc.publisher.facultyFacultad de Cienciases_ES
dc.publisher.programMaestría en Bioquímicaes_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América*
dc.rights.accesRightsopenAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
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dc.subjectpéptidos codificados ribosomalmente, lantibióticos, Bacillus subtilis, Pseudomonas extremaustralis, Escherichia coli, Staphylococcus aureus.es_ES
dc.subject.keywordribosomally coded peptides, lantibiotics, Bacillus subtilis, Pseudomonas extremaustralis, Escherichia coli, Staphylococcus aureuses_ES
dc.titleCaracterización de bacteriocinas de Bacillus subtilis ATCC 6633 y Pseudomonas extremaustralis CMPUJ U515 en la perspectiva de control hacía patógenos humanoses_ES
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersiones_ES
dc.type.spaTesis y disertaciones (Maestría y/o Doctorado)es_ES
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