Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa)

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dc.contributor.advisorMartinez Pachón, Dianaspa
dc.contributor.advisorVanegas Guerrero, Javierspa
dc.contributor.authorPaez Almanza, Valentinaspa
dc.creator.cedula11821819019spa
dc.date.accessioned2023-01-17T16:52:57Z
dc.date.available2023-01-17T16:52:57Z
dc.date.issued2022-12-13spa
dc.description.abstractThe presence and persistence of pharmaceuticals in the food chain, especially in food crops, can have negative effects on human health and the environment. Acetaminophen in particular is a significant environmental pollutant due to its widespread use. The objective of this work was to determine the degradation of acetaminophen (ACE) by endophytic bacteria from lettuce from 3 municipalities of the Sabana de Bogotá (Cota, Mosquera and Funza). For this purpose, lettuce endophytes were selected in minimal salt medium containing acetaminophen, drug degradation was performed by HPLC, and bacteria were identified by 16s rRNA gene sequencing. The bacteria Klebsiella sp L1cp and Klebsiella pneumoniae L2cp use acetaminophen as a carbon and energy source for their growth. Strains L1cp and L2cp degraded 20% and 70% of acetamiphone at 50 mg/l in 60 h. Glucose addition affected ACE degradation, with L1cp degradation increased by 33% and L2cp degradation decreased by 53%. These results suggest the potential of lettuce endophytic bacteria to degrade acetaminophen and a possible mechanism of protection or detoxification of exogenous substances in plants.eng
dc.description.abstractLa presencia y persistencia de productos farmacéuticos en la cadena alimentaria, especialmente en los cultivos alimentarios, puede tener efectos negativos sobre la salud humana y el medio ambiente. El acetaminofén en particular es un contaminante ambiental importante debido a su uso generalizado. El objetivo de este trabajo fue determinar la degradación del acetaminofén (ACE) por bacterias endófitas provenientes de lechuga de 3 municipios de la Sabana de Bogotá (Cota, Mosquera y Funza). Para este propósito, los endófitos de lechuga se seleccionaron en medio mínimo de sal que contenía acetaminofén, la degradación del fármaco se realizó por HPLC y las bacterias se identificaron por secuenciación del gen 16s rRNA. Las bacterias Klebsiella sp L1cp y Klebsiella pneumoniae L2cp utilizan acetaminofén como fuente de carbono y energía para su crecimiento. Las cepas L1cp y L2cp degradaron el 20 % y el 70 % del acetaminofen a 50 mg/l en 60 h. La adición de glucosa afectó la degradación de la ACE, ya que la degradación de L1cp aumentó en un 33 % y la de L2cp disminuyó en un 53 %. Estos resultados sugieren el potencial de las bacterias endófitas de la lechuga para degradar el acetaminofén y un posible mecanismo de protección o detoxificación de sustancias exógenas en las plantas.spa
dc.description.degreelevelPregradospa
dc.description.degreenameBioquímico(a)spa
dc.description.degreetypeInvestigaciónspa
dc.description.notesPresencialspa
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dc.identifier.instnameinstname:Universidad Antonio Nariñospa
dc.identifier.reponamereponame:Repositorio Institucional UANspa
dc.identifier.repourlrepourl:https://repositorio.uan.edu.co/spa
dc.identifier.urihttp://repositorio.uan.edu.co/handle/123456789/7383
dc.language.isospaspa
dc.publisherUniversidad Antonio Nariñospa
dc.publisher.campusBogotá - Circunvalarspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.programBioquímicaspa
dc.rights.coarAcceso abiertospa
dc.subjectDegradaciónes_ES
dc.subjectbacterias endófitases_ES
dc.subjectparacetamol, lechugaes_ES
dc.subject.ddc572es_ES
dc.subject.keywordDegradationes_ES
dc.subject.keywordendophytic bacteriaes_ES
dc.subject.keywordacetaminophen, lettuce.es_ES
dc.titleDegradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa)es_ES
dc.typeTrabajo de grado (Pregrado y/o Especialización)spa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audienceEspecializadaspa
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