Evaluación de la unión de un péptido marcador de neuroinflamación con diferentes proteínas blanco y su conjugación con nanopartículas superparamagnéticas

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dc.contributor.advisorLosada Barragán, Mónicaspa
dc.contributor.advisorLlamosa Pérez, Danielspa
dc.contributor.authorMedina Castillo, Yehidi Juliethspa
dc.creator.cedula1030649417spa
dc.date.accessioned2021-02-18T18:33:52Z
dc.date.available2021-02-18T18:33:52Z
dc.date.issued2020-11-18spa
dc.descriptionInternaes_ES
dc.description.abstractNeuroinflammation is characterized by presenting alterations in the blood-brain barrier, and activation of glial cells, leading to brain tissue alterations generating a pathology, this has led to the need to improve the magnetic resonance imaging technique that allows understanding the development and progression of alterations of the blood-brain barrier in real time, under neuroinflammatory conditions. As an alternative to this solution, it is proposed to establish the binding of a neuroinflammation marker peptide with its target protein and its conjugation with superparamagnetic nanoparticles. The results show that the magnetic nanoparticles have a spherical shape with a size of 54 ± 10 nm, the chemical composition Fe @ SiO2 and a magnetic hysteresis curve indicating a superparamagnetic behavior; The evaluation of the binding of peptide-88 with its possible receptor protein showed a greater binding between peptide-88 with laminin β1, likewise the binding of peptide-88 with the nanoparticles was studied, demonstrating a greater binding unlike the target. The present study showed that by establishing the functionalization of superparamagnetic nanoparticles conjugated to a neuroinflammation biomarker peptide, they can be used for early diagnosis and real-time evolution of the mechanisms triggered under neuroinflammatory conditions.eng
dc.description.abstractLa neuroinflamación es caracterizada por presentar alteraciones en la barrera hematoencefálica, y activación de las células glía, conduciendo a eventos patológicos, esto ha llevado a la necesidad de mejorar la técnica de imagen por resonancia magnética que permitan comprender el desarrollo y progresión de las alteraciones de la barrera hematoencefálica en tiempo real, bajo condiciones neuroinflamatorias. Como una alternativa a esta solución, se propone establecer la unión de un péptido marcador de neuroinflamación con su proteína blanco y su conjugación con nanopartículas superparamagnéticas. Los resultados muestran que las nanopartículas magnéticas presentan una forma esférica con un tamaño 54 ± 10 nm, la composición química Fe@SiO2 y una curva de histéresis magnética indicando un comportamiento superparamagnético; la evaluación de la unión del péptido-88 con su posible proteína receptora mostró una mayor unión entre el peptido-88 con laminina β1, igualmente se estudió la unión del péptido-88 con las nanopartículas demostrando una mayor unión a diferencia del blanco. El presente estudio evidenció que al establecer la funcionalización de nanopartículas superparamagnéticas conjugadas a un péptido biomarcador de neuroinflamación, pueden ser utilizadas para el diagnóstico temprano y evolución en tiempo real de los mecanismos desencadenados bajo condiciones neuroinflamatorias.spa
dc.description.degreelevelPregradospa
dc.description.degreenameBioquímico(a)spa
dc.description.funderCT-672-2018, código 123377757091es_ES
dc.description.notesPresencialspa
dc.description.sponsorshipUAN Proyectos de ciencia, tecnología, innovación y creación artísticaspa
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dc.identifier.instnameinstname:Universidad Antonio Nariñospa
dc.identifier.reponamereponame:Repositorio Institucional UANspa
dc.identifier.repourlrepourl:https://repositorio.uan.edu.co/spa
dc.identifier.urihttp://repositorio.uan.edu.co/handle/123456789/1494
dc.language.isospaspa
dc.publisherUniversidad Antonio Nariñospa
dc.publisher.campusBogotá - Circunvalarspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.programBioquímicaspa
dc.rightsAcceso abierto
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subjectNeuroinflamación, barrera hematoencefálica, nanopartículas superparamagnéticas, péptido biomarcador, proteínas blanco, cultivos in vitro.es_ES
dc.subject.keywordNeuroinflammation, blood-brain barrier, superparamagnetic nanoparticles, biomarker peptide, target proteins, in vitro cultures.es_ES
dc.titleEvaluación de la unión de un péptido marcador de neuroinflamación con diferentes proteínas blanco y su conjugación con nanopartículas superparamagnéticases_ES
dc.typeTrabajo de grado (Pregrado y/o Especialización)spa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1fspa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
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