Identificación de sitios alostéricos en proteínas de Homo sapiens que interactúan con la molécula de ATP mediante la herramienta Deacon Active Site Profiler (DASP3)

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dc.contributor.advisorDuarte González, Mario Enriquespa
dc.contributor.authorGonzález Rosas, Adriana Camilaspa
dc.date.accessioned2021-03-02T15:15:48Z
dc.date.available2021-03-02T15:15:48Z
dc.date.issued2020-07-21spa
dc.description.abstractActive proteins and allosteric sites are distinguished in proteins. The latter have their binding site within the enzyme in a different place from the active site. Its importance lies in the contribution it makes in the inhibition and / or activation of its biological function, this, through molecules (ligands) that act as allosteric modulators, being a basis for the design of drugs, since they provide fewer adverse effects than traditional ones. (active regulators). One of the main molecules that can act as an allosteric regulator is adenosine triphosphate (ATP), since it is essential for obtaining cellular energy. Among the public databases that have information on the structure of proteins, is the National Center for Biotechnology Information (NCBI), there are 1,364,990 proteins in Homo sapiens, some of which have not been studied and therefore it is not known if there is an allosteric site, nor its molecular position. In the work that follows, the creation of an allosteric site profile (ASP) is presented, from three proteins and with the help of the Deacon Active Site Profiler 3 tool (DASP3), which allows identify active sites by creating an active site profile; these proteins, through the reviewed literature, are known allosteric site in interaction with the ATP ligand.eng
dc.description.abstractEn las proteínas se distinguen los sitios activos y los sitios alostéricos. Estos últimos poseen su sitio de unión dentro de la enzima en un lugar diferente al del sitio activo. Su importancia radica en la contribución que realiza en la inhibición y/o activación de su función biológica, esto, mediante moléculas (ligandos) que actúan como moduladores alostéricos, siendo una base para el diseño de fármacos, pues proporcionan menos efectos adversos que los tradicionales (reguladores activos). Una de las principales moléculas que puede actuar como regulador alostérico es el adenosín trifosfato (ATP), ya que es fundamental para la obtención de energía celular. Entre las bases de datos públicas que cuentan con información sobre la estructura de las proteínas, se encuentra el National Center for Biotechnology Information (NCBI), allí existen 1’364.990 proteínas en Homo sapiens, algunas de estas no han sido estudiadas y por tanto no se conoce si existe un sitio alostérico, ni su posición molecular. En el trabajo que se desarrolla a continuación, se presenta la creación de un perfil de sitio alostérico (ASP), a partir de tres proteínas y con la ayuda de la herramienta Deacon Active Site Profiler 3 (DASP3), que permite identificar sitios activos a partir de la creación de un perfil de sitio activo; a dichas proteínas, por medio de la literatura revisada, se les conoce sitio alostérico en interacción con el ligando ATP.spa
dc.description.degreelevelPregradospa
dc.description.degreenameIngeniero(a) Biomédico(a)spa
dc.description.funderCosto del proyecto $ 2’260.000. Financiación propia $ 1’010.000. Financiación UAN $ 1’250.000.es_ES
dc.description.notesPresencialspa
dc.description.sponsorshipOtrospa
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dc.identifier.instnameinstname:Universidad Antonio Nariñospa
dc.identifier.reponamereponame:Repositorio Institucional UANspa
dc.identifier.repourlrepourl:https://repositorio.uan.edu.co/spa
dc.identifier.urihttp://repositorio.uan.edu.co/handle/123456789/2216
dc.language.isospaspa
dc.publisherUniversidad Antonio Nariñospa
dc.publisher.campusBogotá - Surspa
dc.publisher.facultyFacultad de Ingeniería Mecánica, Electrónica y Biomédicaspa
dc.publisher.programIngeniería Biomédicaspa
dc.rightsAcceso abierto
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subjectProteínases_ES
dc.subjectSitio alostéricoes_ES
dc.subjectATPes_ES
dc.subjectBioinformáticaes_ES
dc.subjectDASP3es_ES
dc.subjectDockinges_ES
dc.subject.keywordProteinses_ES
dc.subject.keywordAllosteric Sitees_ES
dc.subject.keywordATPes_ES
dc.subject.keywordBioinformaticses_ES
dc.subject.keywordDASP3es_ES
dc.subject.keywordDockinges_ES
dc.titleIdentificación de sitios alostéricos en proteínas de Homo sapiens que interactúan con la molécula de ATP mediante la herramienta Deacon Active Site Profiler (DASP3)es_ES
dc.typeTrabajo de grado (Pregrado y/o Especialización)spa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1fspa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
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