Identificación por inmunofluorescencia de la expresión de proteínas estructurales del epitelio en células madre del folículo dental prueba piloto

Abstract

The regeneration of the oral mucosa lost due to oral pathologies is a challenge, since that the affected oral tissues need to be restored to their original architecture. The cells of the dental follicle, are presented as an attractive option for the regeneration of tissues of the oral cavity. Previously, the authors have used stem cells obtained of oral tissues, investigating their regenerative potential. The purpose of the present study is identify the expression of typical epithelial proteins Cytokeratin 8, Desmoglein, and Desmoplaquin in stem cells obtained from dental follicle and in future studies its potential application in the regeneration of oral mucosa. METHODS Previous consent, informed consent and institutional ethical endorsement, were obtained dental germs of the third molar in the crown formation stage (n = 5), in patients 14 years old) the samples were stored and processed for the application of techniques of cell culture, microscopic analysis evaluating cell viability, flow cytometry to perform the cellular characterization and later the application of immunohistochemistry, for subsequent identification of protein expression profiles typical of the epithelium in dental follicle tissue. RESULTS The dental follicle stem cell cultures showed adherence to the surface of the culture bottle, cell proliferation, allowing several passages to be made; this allowed evaluate the primary culture obtained, which was characterized by the presentation of several parameters: proliferation rate and expression of typical stromal cell markers mesenchymal (MSC with adequate cell viability, observing in the results the expression of the differentiation markers of the cells and the remarkable expression of the Typical proteins of the epithelium in a greater proportion Desmoglein. CONCLUSIONS The preliminary results obtained in this pilot test provided evidence immunohistochemistry of the expression of typical proteins of the epithelium Cytokeratin 8, Desmoglein and Desmoplaquine, obtained from dental follicle tissue, a result that can be relevant for tissue engineering research, constituting a useful complement for use as a structural alternative for the generation of oral tissues or mucosa oral.