Sistema de recuperación de biomasa a escala de laboratorio mediante filtración tangencial

Abstract

A new functional prototype of a scale bioreactor coupled to a low cost cross-flow filtration system to separate and obtain products of biotechnological interest (biomass). The model has the coupling of a mechanism for measuring temperature, optical absorption and pressure that allow to establish the growth of microorganism cultures in a automated. Development through the design stages links the continuity of experimentation on the growth of microorganism cultures (Saccharomyces cerevisiae), the manufacture of molds and parts from three-dimensional printing (3D), cured with silicone rubber and polyester resin. Integration of elements electronics coupled to software for condition control and recording of behavior of the variables. Thanks to the preliminaries in the elaboration of pieces for the scale bioreactor suitable for sterilization processes and sensor attachment Electronic growth tests were performed from optical measurements and Of temperature; Along with this, it was possible to establish the filtration capacity within the system of flow and pressure relationship. The results obtained show the growth inside the bioreactor at scale of yeast in a homogeneous way. The pressure system succeeded in establishing the behavior under different conditions, with a linear trend in the first 30 mL of sample without the application of prefiltration treatment and shaking; he same linear behavior at 34 mL when the sample passes through the prefilter and shaking and 28 mL of sample when the membrane is washed with hot water coupling improvements in trials. Finally the relationship you have each of the system improvement conditions, making the system have the optimal conditions for the treatment of samples and the washing of membranes.