Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Arenas Suarez, Nelson EnriqueGalindo Morales, Fredy Gonzalo2023-07-312023-07-312023-05-24http://repositorio.uan.edu.co/handle/123456789/8343The increase in agricultural crops has brought with it the increase in the use of insecticides, however, its application has collaterally impacted pollinating insects. Insecticides induce oxidative stress in bees, but the reason why it occurs has not yet been fully elucidated, which is why this study sought to describe the interactions between the insecticide imidacloprid with catalase, glutathione peroxidase and superoxide dismutase using molecular docking, as well as evaluating the expression changes of proteins related to oxidative stress of protein extracts from A. mellifera.El incremento de los cultivos agrícolas ha traído consigo el aumento del uso de insecticidas, sin embargo, su aplicación ha impactado colateralmente a insectos polinizadores. Los insecticidas inducen estrés oxidativo en abejas, mas, la razón por la cual se presenta, aún no se dilucida completamente, es por ello, que en este estudio se buscó describir las interacciones entre el insecticida imidacloprid con catalasa, glutatión peroxidasa y superóxido dismutasa mediante acoplamiento molecular, así como evaluar los cambios de expresión de las proteínas relacionadas con estrés oxidativo de extractos proteicos de A. mellifera.spaAcceso abiertoAbeja, imidacloprid, pesticidas, proteómica, acoplamiento molecular.imidaclopridpesticidasproteómicaacoplamiento molecular57438.23 G158cTrabajo de grado (Pregrado y/o Especialización)Beeimidaclopridproteomicsmolecular dockingpesticidesinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Aguiar, J. M. R. B. V., Nocelli, R. C. F., Giurfa, M., & Nascimento, F. S. (2023). Neonicotinoid effects on tropical bees: Imidacloprid impairs innate appetitive responsiveness, learning and memory in the stingless bee Melipona quadrifasciata. Science of The Total Environment, 877,162859. https://doi.org/10.1016/J.SCITOTENV.2023.162859Aslam, B., Basit, M., Nisar, M. A., Khurshid, M., & Rasool, M. H. (2017). Proteomics: Technologies and Their Applications. Journal of Chromatographic Science, 55(2), 182–196. https://doi.org/10.1093/CHROMSCI/BMW167Balieira, K. V. B., Mazzo, M., Bizerra, P. F. V., Guimarães, A. R. de J. S., Nicodemo, D., & Mingatto, F. E. (2018). Imidacloprid-induced oxidative stress in honey bees and the antioxidant action of caffeine. Apidologie, 49(5), 562–572. https://doi.org/10.1007/S13592-018-0583-1/TABLES/3Blum, M., Chang, H. Y., Chuguransky, S., Grego, T., Kandasaamy, S., Mitchell, A., Nuka, G., Paysan-Lafosse, T., Qureshi, M., Raj, S., Richardson, L., Salazar, G. A., Williams, L., Bork, P., Bridge, A., Gough, J., Haft, D. H., Letunic, I., Marchler-Bauer, A., … Finn, R. D. (2021). The InterPro protein families and domains database: 20 years on. Nucleic Acids Research, 49(D1), D344–D354. https://doi.org/10.1093/NAR/GKAA977Boily, M., Sarrasin, B., DeBlois, C., Aras, P., & Chagnon, M. (2013). Acetylcholinesterase in honey bees (Apis mellifera) exposed to neonicotinoids, atrazine and glyphosate: laboratory and field experiments. Environmental Science and Pollution Research International, 20(8), 5603–5614.https://doi.org/10.1007/S11356-013-1568-2Brandt, A., Gorenflo, A., Siede, R., Meixner, M., & Büchler, R. (2016). The neonicotinoids thiacloprid, imidacloprid, and clothianidin affect the immunocompetence of honey bees (Apis mellifera L.). Journal of Insect Physiology, 86, 40–47. https://doi.org/10.1016/J.JINSPHYS.2016.01.001Buszewski, B., Bukowska, M., Ligor, M., & Staneczko-Baranowska, I. (2019). A holistic study of neonicotinoids neuroactive insecticides—properties, applications, occurrence, and analysis. Environmental Science and Pollution Research, 26(34), 34723–34740. https://doi.org/10.1007/S11356-019-06114-W/TABLES/3Casida, J. E., & Durkin, K. A. (2013). Neuroactive insecticides: Targets, selectivity, resistance, and secondary effects. Annual Review of Entomology, 58, 99–117. https://doi.org/10.1146/annurevento-120811-153645Castellano, I., Cecere, F., De Vendittis, A., Cotugno, R., Chambery, A., Di Maro, A., Michniewicz, A., Parlato, G., Masullo, M., Avvedimento, E. V., De Vendittis, E., & Ruocco, M. R. (2009). Rat mitochondrial manganese superoxide dismutase: Amino acid positions involved in covalent modifications, activity, and heat stability. Biopolymers, 91(12), 1215–1226. https://doi.org/10.1002/BIP.21208Catae, A. F., Roat, T. C., Pratavieira, M., Silva Menegasso, A. R. da, Palma, M. S., & Malaspina, O. (2017). Exposure to a sublethal concentration of imidacloprid and the side effects on target and nontarget organs of Apis mellifera (Hymenoptera, Apidae). Ecotoxicology 2017 27:2, 27(2), 109–121. https://doi.org/10.1007/S10646-017-1874-4instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/