Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Jaimes, GustavoAlfonso, CamiloMayorga Morales, Ana VeronicaRojas Castro, Yuly Daniela2021-03-062021-03-062020-05-29http://repositorio.uan.edu.co/handle/123456789/2724The regeneration of the oral mucosa lost due to oral pathologies is a challenge, since that the affected oral tissues need to be restored to their original architecture. The cells of the dental follicle, are presented as an attractive option for the regeneration of tissues of the oral cavity. Previously, the authors have used stem cells obtained of oral tissues, investigating their regenerative potential. The purpose of the present study is identify the expression of typical epithelial proteins Cytokeratin 8, Desmoglein, and Desmoplaquin in stem cells obtained from dental follicle and in future studies its potential application in the regeneration of oral mucosa. METHODS Previous consent, informed consent and institutional ethical endorsement, were obtained dental germs of the third molar in the crown formation stage (n = 5), in patients 14 years old) the samples were stored and processed for the application of techniques of cell culture, microscopic analysis evaluating cell viability, flow cytometry to perform the cellular characterization and later the application of immunohistochemistry, for subsequent identification of protein expression profiles typical of the epithelium in dental follicle tissue. RESULTS The dental follicle stem cell cultures showed adherence to the surface of the culture bottle, cell proliferation, allowing several passages to be made; this allowed evaluate the primary culture obtained, which was characterized by the presentation of several parameters: proliferation rate and expression of typical stromal cell markers mesenchymal (MSC with adequate cell viability, observing in the results the expression of the differentiation markers of the cells and the remarkable expression of the Typical proteins of the epithelium in a greater proportion Desmoglein. CONCLUSIONS The preliminary results obtained in this pilot test provided evidence immunohistochemistry of the expression of typical proteins of the epithelium Cytokeratin 8, Desmoglein and Desmoplaquine, obtained from dental follicle tissue, a result that can be relevant for tissue engineering research, constituting a useful complement for use as a structural alternative for the generation of oral tissues or mucosa oral.ANTECEDENTES La regeneración de la mucosa oral perdida a causa de las patologías orales es un desafío, ya que los tejidos orales afectados necesitan ser restaurados a su arquitectura original. Las células madre del folículo dental, se presentan como una opción atractiva para la regeneración de los tejidos de la cavidad bucal. Anteriormente, los autores han utilizado células madre obtenidas de tejidos orales, investigando su potencial regenerativo. El propósito del presente estudio es identificar la expresión de las proteínas típicas del epitelio Citoqueratina 8, Desmogleína, y Desmoplaquina en células madre obtenidas a partir de folículo dental y en estudios futuros su potencial aplicación en la regeneración de mucosa oral. MÉTODOS Previo consentimiento, asentimiento informado y aval ético institucional, se obtuvieron gérmenes dentales del tercer molar en la etapa de formación de la corona (n = 5), en pacientes de 14 años de edad) las muestras fueron almacenadas y procesadas, para aplicación de técnicas de cultivo celular, análisis microscópico evaluando la viabilidad celular, citometría de flujo para realizar la caracterización celular y posteriormente la aplicación de técnicas de inmunohistoquímica, para posterior identificación de los perfiles de expresión de las proteínas típicas del epitelio en tejido folículo dental. RESULTADOS Los cultivos de células madre de folículo dental presentaron adherencia a la superficie del frasco de cultivo, proliferación celular, permitiendo realizar varios pasajes; esto permitió evaluar el cultivo primario obtenido, que se caracterizó por la presentación de varios parámetros: tasa de proliferación y expresión de los marcadores típicos de células del estroma mesenquimatoso (MSC con adecuada viabilidad celular, observándose en los resultados la expresión de los marcadores de diferenciación de las células y la expresión notable de la proteínas típicas del epitelio en mayor proporción Desmogleína. CONCLUSIONES Los resultados preliminares obtenidos en esta prueba piloto proporcionaron evidencia inmunohistoquímica de la expresión de las proteínas típicas del epitelio Citoqueratina 8, Desmogleína y Desmoplaquina, obtenidas de tejido folículo dental, resultado que puede ser relevante para las investigaciones de ingeniería tisular, constituyendo un complemento útil para su utilización como alternativa estructural para la generación de tejidos orales o mucosa oral.spaAcceso a solo metadatosMucosa OralCélulas MadreFolículo DentalTrabajo de grado (Pregrado y/o Especialización)Oral MucosaStem cellDental Follicleinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cb1. Menicanin D BP. Identification of a common gene expression signature associated with immature clonal mesenchymal cell populations derived from bone marrow and dental tissues. Stem Cells Dev. 2010 Oct;):1501-10. doi: 10.1089/scd.2009.0492.; 19(10) 1501-1510.2. Saito MT SK. 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