Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Malagon Bernal, Edwin AndresCandela Londoño, Maria Fernanda2021-02-182021-02-182020-11-20http://repositorio.uan.edu.co/handle/123456789/1496InternaInhibition of pancreatic lipase with Orlistat was used to show the correlation between experimental procedures and computational simulations. Both methods are important to understand the kinetics and the inhibition mechanism, and they complement each other in terms of the scope of the results obtained.The enzymatic activity of porcine pancreatic lipase was evaluated by determining kinetic parameters, such as the Michaelis constant- Menten and maximum speed, values corresponding to 61mM and 74µM / min, respectively. Orlistat inhibition tests were performed to obtain Lineweaver-Burk and Dixon graphs, complementary tools with which competitive inhibition was determined. In addition, a molecular coupling was included, to visualize the enzyme-Orlistat binding, along with a lipase sequence analysis to compare the structure of the enzyme in different organisms.La inhibición de la lipasa pancreática con Orlistat se utilizó para mostrar la correlación entre procedimientos experimentales y simulaciones computacionales. Ambos métodos son importantes para entender la cinética y el mecanismo de inhibición, y se complementan en cuanto al alcance de los resultados obtenidos La actividad enzimática de la lipasa pancreática porcina se evaluó con la determinación de los parámetros cinéticos, tales como la constante de Michaelis-Menten y la velocidad máxima, valores que corresponden a 61mM y 74µM/min, respectivamente. Los ensayos de inhibición con Orlistat se realizaron para obtener las gráficas de Lineweaver-Burk y Dixon, herramientas complementarias con las que se determinó una inhibición competitiva. 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