Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Daza Bolaños, Carmen AliciaPacheco Martínez, Fabián Matías2022-08-222022-08-222022-07-14http://repositorio.uan.edu.co/handle/123456789/6809The study of the identification of biomarkers that are linked to the seminal quality of the bull, are very important when quantifying proteins and sperm cells that determine the fertility of the bovine male. For this reason, a research internship was carried out in the Tecnho Sperm laboratory with the aim of acquiring training in the techniques to evaluate the seminal quality of bovine males from flow cytometry, western blot and real-time PCR, as conventional techniques. That are carried out in laboratories whose purpose is to determine the biomarkers present in the fertility of cattle. Once the techniques were applied, it was observed that they are very effective and precise to evaluate the viability of the semen from the different alterations that originate when the sperm are subjected to thermal stress during the freezing process. The hypothermic storage of bovine semen allows its cryopreservation without antibiotics, but this process is limited by the high sensitivity of sperm at the time of freezing.El estudio de la identificación de los biomarcadores que estén vinculados con la calidad seminal del toro, son muy importantes al momento de cuantificar proteínas y células espermáticas que determinen la fertilidad del macho bovino. Por tal motivo se realizó una pasantía investigativa en el laboratorio Tecnho Sperm con el ánimo de adquirir un entrenamiento en las técnicas para evaluar la calidad seminal del macho bovino a partir de la citometría de flujo, western blot y PCR en tiempo real, como técnicas convencionales que se realizan en los laboratorios que tienen como fin determinar los biomarcadores presentes en la fertilidad del ganado bovino. Una vez aplicadas las técnicas se pudo observar que son muy eficaces y precisas para evaluar la viabilidad del semen a partir de las diferentes alteraciones que se originan al momento de someter los espermatozoides al estrés térmico durante el proceso congelación. El almacenamiento hipotérmico del semen del bovino permite su criopreservación sin antibióticos, pero este proceso está limitado a la alta sensibilidad que tiene los espermatozoides al momento de su congelaciónspaAcceso abiertoPCR en tiempo realcitometría de flujoviabilidadcélulas espermáticasTrabajo de grado (Pregrado y/o Especialización)real-time PCRFlow cytometryviabilitysperm cellsinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Boe-Hansen, G. B., & Satake, N. (2019). An update on boar semen assessments by flow cytometry and CASA. Theriogenology, 137, 93–103. https://doi.org/10.1016/j.theriogenology.2019.05.043Llavanera, M., Delgado-Bermúdez, A., Fernández-Fuertes, B., Recuero, S., Mateo, Y., Bonet, S., Barranco, I., & Yeste, M. (2019). GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm. Journal of Animal Science and Biotechnology, 10(1), 1–11. https://doi.org/10.1186/s40104-019-0370-5Llavanera, M., Delgado-Bermúdez, A., Mateo-Otero, Y., Padilla, L., Romeu, X., Roca, J., Barranco, I., & Yeste, M. (2020). Exploring seminal plasma GSTM3 as a quality and in vivo fertility biomarker in pigs—relationship with sperm morphology. Antioxidants, 9(8), 1–14. https://doi.org/10.3390/antiox9080741Meftahi, G. H. (2021). Applications of western blot technique : From bench to bedside. March, 1–9. https://doi.org/10.1002/bmb.21516Memili, E., Moura, A. A., & Kaya, A. (2020). Metabolomes of sperm and seminal plasma associated with bull fertility. Animal Reproduction Science, March, 106355. https://doi.org/10.1016/j.anireprosci.2020.106355Menezes, E. S. B., Badial, P. R., El Debaky, H., Husna, A. U., Ugur, M. R., Kaya, A., Topper, E., Bulla, C., Grant, K. E., Bolden-Tiller, O., Moura, A. A., & Memili, E. (2020). Sperm miR15a and miR-29b are associated with bull fertility. Andrologia, 52(1), 1–11. https://doi.org/10.1111/and.13412Purdy, P. H., Graham, J. K., & Azevedo, H. C. (2021). Evaluation of boar and bull sperm capacitation and the acrosome reaction using flow cytometry. Animal Reproduction Science, 106846. https://doi.org/10.1016/j.anireprosci.2021.106846Radtke, A., Dieckmann, K., Grobelny, F., Salzbrunn, A., & Oing, C. (2019). Expression of miRNA-371a-3p in seminal plasma and ejaculate is associated with sperm concentration. 469–474. https://doi.org/10.1111/andr.12664Salas-Huetos, A., Blanco, J., Vidal, F., Godo, A., Grossmann, M., Pons, M. C., F-Fernández, S., Garrido, N., & Anton, E. (2015). Spermatozoa from patients with seminal alterations exhibit a differential micro-ribonucleic acid profile. Fertility and Sterility, 104(3), 591–601. https://doi.org/10.1016/j.fertnstert.2015.06.015instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/